Plasmid

Part:BBa_K112248

Designed by: Molly Allen   Group: iGEM08_UC_Berkeley   (2008-10-28)


{pAH57 promoter}{rbs.xis.int!}{rbs.ihfA!}{rbs.ihfB!} inserted into K112950 plasmid

This part is inserted into the K112950 plasmid just before attL1.

This part is in BBb Format. It is flanked by BamHI and BglII sites instead of XbaI and SpeI. More information about the BBb Format is available at:

[http://openwetware.org/wiki/Template:AndersonLab:BBb_Standard BBb Standard Description Page]

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 3066
    Illegal XbaI site found at 4309
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 3066
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal prefix found in sequence at 3066
    Illegal BamHI site found at 3976
    Illegal XhoI site found at 5462
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 3066
    Illegal XbaI site found at 4309
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 3066
    Illegal XbaI site found at 4309
    Illegal NgoMIV site found at 5315
    Illegal AgeI site found at 3714
    Illegal AgeI site found at 3826
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//function/recombination/lambda
Parameters
None